Imaging phosphoinositide dynamics using GFP-tagged protein domains
نویسندگان
چکیده
منابع مشابه
Visualizing cellular phosphoinositide pools with GFP-fused protein-modules.
Inositol phospholipids are well known for their pivotal role in calcium signaling as precursors of important second messengers generated in response to various stimuli. However, over the last 10 years, inositides have also emerged as universal signaling components present in virtually every membrane of eukaryotic cells. These lipids are locally produced and degraded by the numerous inositide ki...
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متن کاملImaging phosphoinositide dynamics in living cells.
To improve our understanding of the important roles played by inositol lipid derivatives in signalling and other cellular processes, it is crucial to measure phosphoinositide concentration changes in individual cells with high spatial and temporal resolution. A number of protein domains that interact with inositol lipids in a specific manner have been identified. Tagged with the green fluoresce...
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ژورنال
عنوان ژورنال: Biology of the Cell
سال: 2005
ISSN: 0248-4900
DOI: 10.1042/bc20040080